Practical strategies for the evaluation of high-affinity protein/nucleic acid interactions
نویسندگان
چکیده
منابع مشابه
Practical strategies for the evaluation of high-affinity protein/nucleic acid interactions
The quantitative evaluation of binding interactions between proteins and nucleic acids is highly sensitive to a variety of experimental conditions. Optimization of these conditions is critical for obtaining high quality, reproducible data, particularly in the context of very high affinity interactions. Here, we discuss the practical considerations involved in optimizing the apparent binding con...
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ژورنال
عنوان ژورنال: Journal of Nucleic Acids Investigation
سال: 2013
ISSN: 2036-7996,2035-6005
DOI: 10.4081/jnai.2013.4646